(KIT) BLOCK-IT ADENO EXP SYST - 10624803

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(KIT) BLOCK-IT ADENO EXP SYST - 10624803

Description

Description

  • The pAd/BLOCK-iT-DEST RNAi Gateway Vector can be used for the efficient delivery
  • and transient expression of a short hairpin RNA (shRNA) in vivo from an
  • adenoviral vector. A novel cloning process places an ~50-bp DNA oligonucleotide
  • immediately following a U6 pol III promoter into the BLOCK-iT U6 entry vector.
  • After efficient recombination of the entry vector into the pAd/BLOCK-iT-DEST
  • vector, followed by viral production and transduction, the shRNA driven by the
  • U6 promoter can be transiently expressed in most dividing or non-dividing
  • mammalian cell types. The shRNA generated avoids the hosts defense mechanism and
  • will be effective at producing the RNAi gene knockdown response.The
  • pAd/BLOCK-iT-DEST vector (Figure 1) offers:A promoterless region surrounded by
  • attR sites for efficient recombination with the attL-flanked U6 Gateway entry
  • vector containing the RNAi cassette or any attL-flanked promoter and gene
  • sequenceAll of the required components for efficient adenoviral packaging and
  • delivery of the shRNA of interest. With this vector, transient analysis of gene
  • knockdown in both dividing and non-dividing mammalian cell types and animal
  • models can be achieved. For Research Use Only. All usage must comply with
  • product instructions.

Caractéristiques

  • Marque: INVITROGEN
  • Conditionnement : 1PCS
  • Ancienne référence: W3214C

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