(KIT) BLOCK-IT H1 IND ENTRY VECT - 10769934

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(KIT) BLOCK-IT H1 IND ENTRY VECT - 10769934

Description

Description

  • Take control with inducible RNAi-in any cell type. Take control with inducible
  • RNAi! The BLOCK-iT Inducible H1 RNAi Entry Vector is used to generate stable
  • cells that can be induced to express short hairpin RNA (shRNA). The shRNA leads
  • to an RNAi response that results in down regulating a targeted protein. When
  • introduced into cells expressing the tetracycline repressor (TR) protein, the
  • induction of the RNAi pathway occurs in the presence of tetracycline. When
  • tetracycline is not present, there is almost no change in cellular levels of
  • protein. The BLOCK-iT Inducible H1 RNAi Entry Vector provides a simple,
  • stream-lined approach to cloning shRNA. The BLOCK-iT Inducible H1 RNAi Entry
  • Vector allows you to:Study genes whose removal results in cell deathControl
  • initiation of an RNAi response to match experimental needsStudy a phenotype
  • without activation of compensatory mechanisms that can occur when the shRNA is
  • continually expressedMechanism of shRNA expression from the H1/TO RNAi
  • cassetteAn easy cloning process places a ~50-bp double-stranded DNA
  • oligonucleotide encoding an shRNA immediately downstream of the H1/TO pol III
  • promoter to create an H1/TO RNAi cassette (Figure 1). This H1/TO pol III
  • promoter contains two TetO2 sites for tetracycline-regulated expression.
  • Cellular transcription of the dsDNA produces an shRNA molecule that is processed
  • into short interfering RNA (siRNA) capable of inhibiting expression of the
  • target gene. In cells that do not express the TR protein, the shRNA is

Caractéristiques

  • Marque: INVITROGEN
  • Conditionnement : 1SET
  • Ancienne référence: W3213P

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