(KIT) BLOCK-IT LENTI IND RNAI SYS - 10779934

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(KIT) BLOCK-IT LENTI IND RNAI SYS - 10779934

Description

Description

  • Take control with inducible RNAi-in any cell type. The pLenti4/BLOCK-iT-DEST
  • expression vector provided in the BLOCK-iT Inducible H1 Lentiviral RNAi System
  • can be used to efficiently regulate RNAi in virtually any mammalian cell. Using
  • the BLOCK-iT Inducible H1 Lentiviral RNAi System, long-term inducible shRNA
  • expression is possible. A novel cloning process places a double-immediately
  • following an H1/TO pol III promoter to generate an H1/TO RNAi cassette in an
  • inducible BLOCK-iT H1/TO entry construct. This can be quickly and efficiently
  • recombined from the pENTR/H1/TO entry vector into the pLenti4/BLOCK-iT-DEST
  • vector via a standard Gateway LR recombination reaction. Transfect this
  • construct along with the ViraPower Lentiviral packaging mix into 293FT cells
  • with Lipofectamine 2000 Reagent to produce viral particles capable of
  • transducing any mammalian cell. If the cell expresses the tetracycline repressor
  • (TR) protein, inducible RNAi is possible.The pLenti4/BLOCK-iT-DEST expression
  • vector enables lentiviral delivery and genomic integration of DNA coding for
  • shRNA. Once expressed, the shRNA is processed by cellular machinery and
  • initiates target-specific RNAi. The pLenti4/BLOCK-iT-DEST vector offers:Gateway
  • Technology for efficient recombination of the RNAi cassette from the BLOCK-iT
  • inducible pENTR/H1/TO vectorAll required components for efficient lentiviral
  • packaging, delivery, and integration of the shRNAZeocin selection marker for
  • fast selection of clonal cell lines containing the RNAi cassetteRegulated shRNA

Caractéristiques

  • Marque: INVITROGEN
  • Conditionnement : 5GR
  • Ancienne référence: W3213R

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