(KIT) BLOCK IT LENTI RNAI EXP SYSTEM - 10267232

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(KIT) BLOCK IT LENTI RNAI EXP SYSTEM - 10267232

Description

Description

  • The pLenti6/BLOCK-iT-DEST expression vector provided in the BLOCK-iT Lentiviral
  • RNAi Expression System can be used to efficiently introduce and stably express
  • short hairpin RNA (shRNA) in vivo from a lentiviral vector. A novel cloning
  • process places an ~50-bp DNA oligonucleotide immediately following a U6 pol III
  • promoter into the BLOCK-iT U6 entry vector. The oligonucleotide is designed
  • toexpress RNA that forms a stem-loop structure containing the sense and
  • antisense regions of your target gene of interest. This shRNA is then recombined
  • into the pLenti6/BLOCK-iT-DEST vector. After viral production and transduction,
  • the shRNA driven by the U6 promoter becomes stably integrated as an RNAi
  • cassette. The shRNA generated avoids the hosts defense mechanism and will be
  • effective at producing the RNAi gene knockdown response (Figure 1).The
  • pLenti6/BLOCK-iT-DEST vector (Figure 2) offers:attR sites for efficient
  • recombination with the attL-flanked U6 Gateway entry vector containing the RNAi
  • cassetteAll of the required components for efficient lentiviral packaging and
  • delivery of the shRNA of interestBlasticidin selection marker for fast,
  • efficient selection of stable cell lines expressing the shRNA Using the BLOCK-iT
  • Lentiviral RNAi Expression System, long-term analysis of gene blocking in both
  • dividing and non-dividing mammalian cell types and animal models can be
  • achieved.The BLOCK-iT RNAi U6 Entry Vector Kit allows streamlined cloning of
  • shRNA target sequences for testing in transient experiments. Selected RNAi

Caractéristiques

  • Marque: INVITROGEN
  • Conditionnement : 5GR
  • Ancienne référence: W3214E

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