(KIT) BLOCK IT U6 ENTRY VECTOR KIT - 10535383

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(KIT) BLOCK IT U6 ENTRY VECTOR KIT - 10535383

Description

Description

  • The BLOCK-iT U6 RNAi Entry Vector provides a simple, streamlined approach to
  • cloning short hairpin RNA (shRNA) sequences for testing in transient
  • transfections for RNA interference (RNAi), a preferred technique for analyzing
  • gene downregulation. An easy cloning process places an ~50-bp oligonucleotide
  • DNA immediately following a U6 pol III type promoter (Figure 1). Expression of
  • this RNAi cassette forms a shRNA molecule in the cell that will be processed and
  • act as short interfering RNA (siRNA) that will generate the RNAi effect.Delivery
  • of the U6 RNAi CassetteOnce cloning is complete, the U6 Entry Vector is ready to
  • be used immediately in transient transfections using a reagent such as
  • Lipofectamine 2000. This makes this system ideal for initial shRNA screenings in
  • many mammalian cell types. As an alternative in hard-to-transfect or
  • non-dividing cell types, or to deliver to animal model systems, the RNAi
  • cassette can easily be recombined into a BLOCK-iT Viral RNAi vector. For stable
  • shRNA lentiviral delivery and expression, recombine the BLOCK-iT U6 Entry Vector
  • with the pLenti6/BLOCK-iT RNAi Vector. For transient delivery to challenging
  • cell types using adenoviral transduction, recombine with the pAd/BLOCK-iT RNAi
  • Vector. For Research Use Only. All usage must comply with product instructions.

Caractéristiques

  • Marque: INVITROGEN
  • Conditionnement : 1SET
  • Ancienne référence: W3214F

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